年1月23日 CalcuSyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in. combination-index data generated by CalcuSyn software analyses .. and antagonism, respectively (Source: CalcuSyn manual, Biosoft, ). Manual and Software, Biosoft, Cambridge, U.K., Chou, T.-C. and Hayball , M. CalcuSyn for Windows, Multiple-drug dose-effect analyzer and manual.

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Prined in the U.

The report window will be displayed – this is a standard text window with most of the controls available in Notepad. Command Line Import There are two different types of command-line import, both require a text file. The simplicity of the general equations makes for versatility in their applications.

When report or graph windows are selected, the estimated errors will be added. Import drugs and combinations directly from a text file 3. Click on OK to complete the entry. Constant Ratio Combination Design The most efficient way for experimental design is to choose the combination drugs at their equipotent ratio e. For single drugs, this will be the median plot and the dose-effect plot. It is considered the first line of statistics to measure the conformity of the data with the mass-action law principle when the experimental measurement is assumed to be accurate.

Estimates of accuracy of EDx and CI can be calculated with Monte Carlo simulations or by a highly accurate algebraic estimation algorithm. A condition in which one of two drugs is not effective by itself, but increases the effect of the other drug.

Worked examples The following reports show the summary results for all six examples included with CalcuSyn.

## Computer Software

Non-constant Ratio Combination Design Sometimes, researchers design their drug combinations with random or arbitrary ratios, or vary the concentrations of one drug while keeping the second drug concentration fixed. Graphs Open the relevant window; either the raw data grid, a report window, or a graph window then select File Export. Double click on items in the pane to open them.

Instead, potentiation augmentation, enhancement or inhibition suppression can be determined. Using the Program 27 Entering a Combination Drug As with entering a single drug there are two methods to enter a combination drug: State that the drugs are in constant ratio to each other and enter the ratio of the two drugs to each other e.

When drug 1 and drug 2 yield parallel lines in the median-effect plot and the mixture of the two drugs also yields a parallel line, mutual exclusivity msnual indicated 6,8. The symbols used are explained in the glossary and in detail in the theory section of this manual. To control which ones appear, go to Setup Options cqlcusyn the Main Menu and the dialog illustrated after the table will open. Rotenone Data points entered: The m can be readily determined by the slope of the median-effect plot of Chou Double-click on Raw Data to open the raw data grid to enter drug data.

Using the Program 29 The combinations will then be added to the experiment and shown in the Contents Pane. CalcuSyn 30 Step 3 – Enter the drug name, units and any comments. However, one should realize that an antagonistic action may be desired when toxicity is concerned, i.

Whereas the iso-effective graph in the classic isobol is represented by a straight diagonal line, the iso-effective graph in the conservative isobol is represented by a convex upward curve. A measure of how much the dose of each drug in a synergistic combination may be reduced at a given effect level compared with the doses of each drug alone Application of the Chou-Talalay Method 15 Application of the Chou-Talalay Method Scope of Application Since both the median-effect equation of Chou and the combination-index equation of Chou-Talalay are general equations derived from the massaction law models, their derivations have rigorous mathematical proof and systematic documentation.

First and foremost, you should know it is possible to import data from TXT and XLS files, as well as export it to such documents, so that you can use it in other programs or keep it safe.

### Biosoft: Software for Science

The median-effect principle and the combination index for quantitation of synergism and antagonism. For simplicity, mutual exclusivity is usually calcsyn when more than two drugs are involved in combinations 8,9, If the Monte Carlo option is selected in the Reporting Options dialog then the mean and standard deviation values of CI calculated using the Monte Carlo algorithm are displayed as for the CI simulations above.

Whether or not you have been able to replicate the problem and, if so, the steps necessary to do so. Already used in hundreds of academic labs and pharmaceutical companies, CalcuSyn is indispensable for the study of drug mixtures. Drug manial are widely used manaul multiple drugs affect multiple targets and cell subpopulations. Two drug combination – Toxicity of pyrethins and rotenone to houseflies. Combination of more than Two Drugs For three drug combinations e.

The symbols used in the graphs are as follows: Step 4 – Highlight the dose data in the grid as shown and click the Select menu option. Using the Contents Calchsyn On the left-hand side of the program screen is the contents pane that shows the contents of your experiment see below.

The following examples are based around the sample2. However, if too many effect levels are selected, the isobolograms frequently become messy and difficult to read. Chemotherapeutic synergism, potentiation and antagonism. The estimated standard deviation given is calculated using an approximation described in the appendix: By analyzing the distribution of the CI values the czlcusyn distribution can be estimated.

Assessment of synergistic and antagonistic effect of chemotherapeutic agents in vitro. When the grid is used, data can be processed through wizards which make the software easily accessible to new users. You can only import data from the first sheet of a workbook. Re-arranging equation 2 we get: It must be typed exactly as shown although the case of the letters is not crucial. Ara-C Fraction affected uM 0. Accuracy of Measurement and Conformity to the Mass-action Law The linear correlation coefficient, r, of the calcushn plot should be reasonably good.

Using the Program 47 Data points entered: