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Sonoporation – Wikipedia
Measurement of the acoustics used in sonoporation is listed in terms of mechanical indexwhich quantifies the likelihood that exposure to diagnostic ultrasound will produce an adverse biological effect by a non-thermal action based on pressure. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults.
With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.
Electroporation and Sonoporation in Developmental Biology. European Journal of Echocardiography.
Electroporation and Sonoporation in Developmental Biology by Harukazu Nakamura on Apple Books
Sonoporationelectroporatioj cellular sonicationis the use of sound typically ultrasonic frequencies for modifying the permeability of the cell plasma membrane.
Sonoporation may also be performed with custom-built piezoelectric transducers connected to bench-top function generators and acoustic amplifiers.
My library Help Advanced Book Search. Sonoporation is performed with a dedicated sonoporator. Sonoporation uses microbubbles for significantly enhancing transfection, and in some cases is required for DNA uptake. By using this site, you agree to the Terms of Use and Privacy Policy.
Acoustics Research Letters Online.
Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of these large molecules. From Wikipedia, the free encyclopedia.
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Sonoporation is also being studied for use in targeted Gene therapy in vivoin a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patient’s body. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation.
Introduction of a tetracycline-regulated gene expression system and a transposon It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes.
Electroporation and Sonoporation in Developmental Biology
In ovo electroporation is an epoch-making achievement in the study of developmental biology. Retrieved from ” https: Selected pages Title Page. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. Standard ultrasound medical devices may also be used in some applications.
Sonoporation is under active study for the introduction of foreign genes in tissue culture cells, especially mammalian cells. This page was last edited on 22 Novemberat Views Read Edit View history.