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Moreover, HSAIg, a fusion protein consisting of the extracellular domain of the HSA and the Fc portion of immunoglobulin, drastically ameliorates the clinical sign of EAE even when administrated after self-reactive T cells had been fcx. TPA and phenylephrine stimulate expression of the Glut1 gene. Detection of Ras-GTP in cells extract was performed as described RT-PCR was performed with 1. Global Montello Group Corp.
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This increase was mainly achieved by overexpression of Glut1 and to a minor extent by a decrease in Glut4 expression. Transient transfection experiments with a luciferase reporter under the control of the mouse Glut1 promoter indicated that treatment of myocytes with hypertrophic agonists resulted in increased transcription from the Glut1 promoter occurring between 6 and 48 h following addition of the agonist.
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Inhibition of the phosphatidylinositol 3-kinase pathway reduces hypertrophic Glut1 induction. Interestingly the signaling pathways that are used in different cell types in cfs heart to activate the ERK molecules are different.
Advanced America PetroProducts Inc. Camin Cargo Control, Inc. Although an initial report described PI3K as being a direct target of Ras 32other studies have suggested that PI3K could act upstream of Ras 46 Berkebile Oil Company, Inc. ER-transfected cells were then treated with either 0. Expression of Endogenous Glucose Transporters To evaluate the relative expression of the endogenous glucose transporter genes Glut1 and Glut4 by RT-PCR, we took advantage of regions of structural similarity and differences between the two isoforms Jump to main content or area navigation.
Myocardial cells can use a wide variety of substrates for energy production, including free fatty acids, glucose, lactate, and ketone bodies. To evaluate the relative expression of the endogenous glucose transporter genes Glut1 and Glut4 by RT-PCR, we took advantage of regions of structural similarity and differences between the two isoforms Cells were plated onto glass coverslips coated with gelatin and laminin and left untreated or treated with TPA or PE for 48 h.
We are confident, however, that this is not the case, for the following reasons. Journal of Lipid Research.
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Advanced Fuel Services, Inc. Treatment with PD did not affect base-line expression of either the Glut1 or Glut4 endogenous gene.
Responses Submit a Letter to the Editor. Castrol Heavy Duty Lubricants, Inc. Anhui Weichi Chemical Co. Cells were treated for 48 h and then fixed and stained with fluorescein isothiocyanate-conjugated phalloidin to show filamentous actin. Blots were probed using a rabbit anti-Ha-Ras polyclonal antibody Santa Cruz sc TPA and phenylephrine increase transcription from the Glut1 promoter.
Frontier Performance Lubricants, Fcd. Figure 9 Inhibition of the phosphatidylinositol 3-kinase pathway reduces hypertrophic 90012 induction. For transfection experiments, cells were plated at a density of 2. Furthermore, expression induced by all of these agonists was inhibited by cotransfection with the broad specificity MAP kinase phosphatase CL Vice President Daniel P. However, treatment with Fccs markedly reduced expression of the Glut1 gene induced by either TPA or phenylephrine, without affecting expression of the Glut4 gene Fig.
In addition, active MAP kinase is required for induction of the c- fos and atrial natriuretic factor promoters by phenylephrine 4142and expression of constitutively active MEK1 results in overexpression of hypertrophic genes Dae Yang Industrial Co.
Figure 7 Ras activity is required for induction of the Glut1 promoter in myocytes.
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Colonial Oil Industries, Inc. Blackman Uhler Chemical Fcss. View this article with LENS. Figure 2 TPA and phenylephrine stimulate expression of the Glut1 gene. Cells were then stained with fluorescein isothiocyanate-labeled phalloidin to show filamentous actin. This result suggests that Ras activation is required for transduction of the signal elicited by both TPA and phenylephrine in cardiac myocytes. Falcon Safety Products, Inc.
American Technologies Group, Inc. Advanced Technology Lubricants, Inc. This observation is corroborated by the finding that TPA treatment resulted in activation of Ras in myocytes, but not in fibroblasts see below. Easy Energy Systems, Inc.