Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore. Somatic hybridization by fusion technique is a breakthrough technique that allows the plant generated by an fusk level ploidy. Language Select Language English Indonesian. Pusat Penelitian dan Pengembangan Perkebunan Jln.

Plants with triploid ploidy level usually have sterile fruit seedless. Five random primers, OPD DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al.

First group consisted of somatic hybrids nos.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd

Sidikalang and TT 75 were tested for their genetic variability and relationship. Index of similarity among genotypes were calculated by using NTsys ver. Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan.

The strategy chosen to assemble the triploid tangerine because the technique of protoplast isolation, fusion and regeneration of tangerine with diploid level has been mastered ploidy. Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs. The best enzyme formulations for the isolation of protoplasts from diploid callus and leaves in vitro and protoplast Haploid pollen is macerozim 0.


Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0. OPH 19 and Abi Some features of this site may not work without it. Based on the distribution of phenols and lignin content.

Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus.

Fusi protoplas interspesifik aspergillus Spp

One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus. View original from ejurnal. Email the author Login required. Pre cold treatment for more than 3 days on the response forming callus from pretreatment less than 3 days.

Electronic Theses & Dissertations Universitas Gajah Mada

Jurnal Penelitian Tanaman Industri merupakan publikasi ilmiah primer yang memuat hasil penelitian Seedless citrus improvement can be done by manipulating the level ploidy. Ihe level of resistance of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al proyoplas laboratory of the Research Institute for food Crop Biotechnology, Bogor in November User Username Password Remember me.


Result showed that Java patchouli Girilaya contained phenols and lignin respectively Industrial Crops Research Journal. Evaluation of phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd – Neliti

S Assessment of genetic variability of patchoulli Pogostemon sp. The second group was then classified into 2 minor groups. Phenol and lignin content in the protoplass hybrids were dilferenl in Ihe respective pairs.

Ihe somatic hybrids can be categorized in three group The irst groups 5 clones contained high phenols higher than the average. Download full text Bahasa Indonesia, 6 pages.

Ihe second group 9 clones contained high lignin. Email this article Login required.

The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli Sidikalang and TT 75and the somatic hybrids.

Haploid cell population repeatedly sub cultured every 4 weeks in a double layer media: In the fusion of Girilaya x Sidikalang.