API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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Quality control The overall performance of the system should be monitored by testing ggalerie control strains. Using the results obtained, the indices of the positive reactions are circled. Citrate utilization citrate is the only source of carbon. The sum of these indices in each group of three reactions forms the code number. Each strip consists of 12 different biochemical substrates. The 12A 12E strip should be read at hours.
The following chart gives the expected results on the Microbact System after an hour incubation: Indole is formed from metabolism of tryptophan. Hydrolysis of gelatin is indicated by dispersal of the black particles throughout the well. Green should be regarded as a negative reaction. The 12A strip may be pai alone for identification of oxidase-negative, nitrate-positive glucose fermenters comprising 15 genera and may be useful for screening pathogenic Enterobacteriaceae from enteric and urine specimens or identification of other common isolates.
This code is entered into the computer package. Material Safety Data Sheet. These strips are intended for in vitro use only; for use by qualified laboratory personnel using aseptic techniques and established precautions against microbiological hazards.
When Actinobacillus or Pasteurella spp. Before use, perform an oxidase test on the organism to be identified.
Reactions obtained using the Microbact System may differ from published results using other substrate formulations. Bromothymol blue indicator changes from blue to yellow when the carbohydrate is utilised to form acid.
Identification of Pseudomonas aeruginosa with the API-20E system.
Using a sterile Pasteur pipette, transfer one drop of the peptone water culture into the appropriate volume see Procedure Chart of sterile saline solution 0.
The pH shift indicated by bromothymol blue caused by formation of the specific amine putrescine is greater than that caused by lysine decarboxylation.
Please click on a thumbnail to view the apl size image and open the gallery. Well 8 Indole production – add 2 drops of Indole Kovacs reagent. Microbact Biochemical Identification Kits.
Production of indolepyruvate by deamination of tryptophan. One drop of Nitrate reagent A and 1 drop of Nitrate reagent B is added to the well.
Identification of Pseudomonas aeruginosa with the APIE system.
Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2. After the addition of Nitrate reagents A and B: Evaluate 15 to 30 minutes after the addition of reagents. The 24E solid microplate format contains the 24 substrates contained in the combination of both the 12A and 12B strips. Kit presentation Each kit contains the following: Used materials should be autoclaved, incinerated, or immersed in germicide before disposal.
Thomas, Veterinary Microbiology. Motility and DNase tests are recommended for further speciation of this group. Refer to the Table of Reactions below for the substrates contained in each well, 20d specific reaction principle, and colour changes. Biochemical Characterisics of Enterotoxigenic Aeromonas sp. Incubation Reseal the inoculated rows with the adhesive seal and write the specimen identification number on the end tag with a marker pen.
H 2 S reacts with ferric salts in the medium to form a black precipitate. The lysine and ornithine decarboxylase reactions should be carefully interpreted. Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred.
Strips stored in this manner must be used within 7 days. Liquefaction of gelatin by proteolytic enzymes diffuses the black pigment. Remove the strips or tray from the incubator, peel back the sealing tape. For aid in interpreting reactions, refer to the Table of Reactions. The 12 substrates contained in the 12A strips are available in a solid microplate format, referred to as 12E.
Some bacterial strains may have atypical biochemical reactions due to unusual nutritional requirements or mutations and may be galere to yalerie. A small amount of zinc powder should be added to those wells which exhibit a yellow colour after the addition of the nitrate reagents.
Utilisation of Na malonate at the 20d time that ammonium sulphate is utilised as the nitrogen source produces sodium hydroxide resulting in increased alkalinity and a blue colouration. Gram-stain, colonial morphology, source of isolate, percent probability degree of separationtests against, additional test indications and results, frequency of ID choice and antibioGram.
Bromothymol blue indicates formation of the specific amine cadaverine. The 12B strips can be used alongside the 12E, but in a separate tray.