Genencor International (Danisco A/S) in the US was developing I V, In addition, there was negligible IgG antibody response to the variant interferon-β. Herceptin IgG human antibody were made and transformed into Trichoderma reesei. Genencor International, a Danisco Company. Page. GENENCOR INTERNATIONAL PALO ALTO CA Initial genetic constructs for the Herceptin IgG human antibody were made and transformed into Trichoderma.

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At 37 degrees and a pH of 7. The measurements were carried out in duplicate and are shown in Table 6. In addition to the kDa form, a series of protein bands at approximately,and kDainterpreted as assembled antibody with from one to four glucoamylase molecules attached to the N termini of the individual antibody chains, were observed for An-trast-I data not shown and for An-trast-II and An-Hu1D10 Fig.


For an illustration of this procedure see U. The average proliferation is recorded above for each peptide across the five animals. The chimeric molecules are typically combined with a pharmaceutically acceptable carrier excipient to form a pharmacological composition. T helper epitopes have become common components of synthetic vaccine constructs due to their ability to provide immunological help for both humoral and cell-mediated responses [ 1114 – 16 ].

In certain embodiments, the cysteine to which the targeting moiety is attached is a native cysteine in the enzyme, however, in preferred embodiments the cysteine is a cysteine substituted for a different native amino acid residue in the enzyme.

Enhanced immunogenicity of a functional enzyme by T cell epitope modification

BPN’ YL may possess weak T cell epitopes ogg are below our limit of detection in our cell culture assay.


Based upon the presence of these various extracellular domains the RTKs have been sub-divided into at least 14 different families. Xenopus oocytes into which an RNA encoding the subject receptor is inserted.

Peptides 25 and 75 induced average responses over the 5 animals that reached an S.

By way of further illustration, however, a number of specific, particularly preferred embodiments are discussed below. The term ligand is used to refer to a molecule that specifically binds to another molecule. Guinea venencor T cell epitopes in B. After 15 min, the reactions in 4 for SC-Biotin started to visibly change their color to pale yellow.

Peptide mapping analysis was performed by using the Micromass ProteinLynx 3. Assembly of antibody presumably occurs in the endoplasmic reticulum of A. Serum samples were taken every two weeks and titers were calculated.

Priming of cytotoxic cell responses is necessary for the design of therapeutic cancer vaccines, as well as for viral vaccines. Finally, we show that the variant induces broadly cross-reactive IgG antibodies, and that priming of animals with the variant protein results in higher titers of antigen-specific antibody upon subsequent immunization with the parent enzyme.



Fong Genencor International, Inc. When provided in this manner the kits enable one or ordinary skill in the art to assemble the desired chimeric molecule for a particular use. Naoya Tsurushita Genencor International, Inc. The subtilisins and chymotrypsin related serine proteases have a catalytic triad comprising aspartate, histidine and serine.

The measurements were carried out in duplicate. The branching occurs at the nitrogen atom of a terminal amine group on a preceding generation branch. In a particulary preferred embodiment the fraction is dialyzed against pH 6. The antagonist of claim 1wherein said targeting moiety is joined to said enzyme through the sulfur group on a cysteine.


For these antibodies, genenclr was evident that cleavage of the glucoamylase-antibody fusion proteins was not complete. Such enzymes include, but are not limited to proteases, cellulases, nucleases exo- and endo-amylases, Upases, aldolases, ketolases, glycosidases, and the like.

Characterization of Humanized Antibodies Secreted by Aspergillus niger

This article has been cited by other articles in PMC. In other particularly preferred embodiments, the chimeric molecules of this invention can be used to target and destroy particular preselected molecules whether or not they have a biological activity. The synthesis of the MTS-pyrazole reagent is illustrated by scheme 11 Figure 3.

People with this disease have a deficiency in a major protein, alpha- 1 proteinase inhibitor.

The conjugation can be through the existing R group using other coupling chemistriesor alternatively a sulfhydryl group SH can be introduced linked genemcor the R group and the targeting moiety, derivatized as a methanethiosulfonate reagent, can be coupled, e. This was emulsified at a 1: The polyepitope iyg to DNA vaccination. Finally, animals primed to the variant but boosted with the parent genencir had higher levels of antigen-specific IgG than animals immunized with the parent enzyme alone.

This may be rationalized in terms of the pyrazole moiety adopting a conformation where it bends outside the binding pocket. Kinetic constants determined in duplicate by method of initial rates in 0. In addition, a single immunization with the T cell epitope modified variant GP is sufficient to induce higher levels of parent-enzyme-specific IgG after subsequent immunizations with the unmodified parent enzyme.