Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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We hydrocarbonoclaatic not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. Society of Petroleum Engineers. Obligate marine hydrocarbon bacteria genera include Marinobacter, Marinobacterium, Alcanivorax, Neptunomonas, Thalassolituus, Cycloclasticus, Oleispira and Oleiphilus. This research is aimed at isolating hydrocarbonoclastic bacteria from petroleum refinery effluent and also assessing their bioremediating potentials in hydrocarbon cleanup programmes.

Consortia of HCB can have broad spectrum hydrocarbonoclastic potential than individual strains. The results of sequencing of the nifH gene bands in Fig. Microbiological nitrogen fixation is an activity that requires large amounts of energy.

Petroleum microbiology – Wikipedia

This finding is similar to the study carried out by Kafilzadeh et al. Identities of epilithic hydrocarbon-utilizing diazotrophic bacteria from the Arabian Gulf coasts, and their potential for oil bioremediation without nitrogen supplementation. Retrieved from hydrocarbonoclasti https: The results obtained from the identification were compared with Bergey Manual of Bacteriology Holt, This result provides conclusive evidence for the ability of hydrocarbonoclastic bacteria to fix nitrogen in situ.

Five hydrocarbonoclatic bacteria isolated were Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, Serretia marcescens and Streptomyces sp.

Isolation of Hydrocarbonoclastic Bacteria 2. Identification of Hydrocarbonoclastic Bacteria.


Pseudomonas stutzeri partial nifH gene for putative nitrogenase iron protein, strain Gr Marinobacterium jannaschii and Oleispira batceria are psychrophilic and halotolerant HCBs. Alcanivorax borkumensis and A. Sinorhizobium meliloti partial nifH gene for nitrogenase reductase, strain Gr Abstract Eighty-two out of the hydrocarbonoclastic bacterial species that have been already isolated from oil-contaminated Kuwaiti sites, characterized by 16S rRNA nucleotide sequencing, and preserved in our private culture collection, grew successfully in a mineral medium free of any nitrogenous compounds with oil vapor as the sole carbon source.

The gene amplicons were subsequently separated and detected by gel electrophoresis.

Potential Hydrocarbonoclastic Bacteria

However, in the absence of NaNO 3active growth only occurred when the top space of the cultures contained nitrogen gas. Petroleum, crude oil or oil and hydrocarbons are sometimes used synonymously.

Enhancement of rhizosphere of crop plants raised in oily sand hydrocarbonoclastlc hydrocarbon-utilizing bacteria capable of hydrocarbon consumption in nitrogen free media.

They are rarely present in unpolluted areas! The experimental set bacteroa was left to stand for ten days at room temperature and the COD was measured using reflux digestion method at two days intervals Usman et al.

Hydrocarbons are compounds whose structures are made up of hydrogen and carbon atoms. Different species of Marinobacter like alkaliphilus, aquaeolei, articus, hydrocarbonoclasticus, maritimus and squalenivorans are strictly HCB. The percentage reduction of the organic components in treatments having Pseudomonas aeruginosa, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris, Streptomyces spp.

It constitutes the primary mechanism for the elimination of hydrocarbons from contaminated sites by natural existing populations of microorganisms.

Residual crude oil was extracted with three successive mL portions of pentane. In this work, a collection of strains, adapted to grow on minimal medium supplemented with crude oil, baacteria obtained from contaminated sediments and seawater from hydrocarbonoclaastic refinery harbor of the Bizerte coast in the North of Tunisia. From Wikipedia, the free encyclopedia. Petroleum effluent is discharged into River Rido and this poses a threat to the communities that utilize the water downstream of River Rido, the ultimate recipient of the effluent.


Petroleum is composed of straight or branched saturated aliphatic, alicyclic, aromatic and unsaturated olefinic hydrocarbons. Open in a separate window.

Petroleum microbiology

Growth curves of bacterial species in the presence and absence of NaNO 3 and nitrogen gas. This page was last edited on 26 Juneat On the other hand, these bacteria only grew and consumed crude oil in hdrocarbonoclastic NaNO 3 -free medium when the top space gas contained nitrogen.

Study of nitrogen fixation in microbial communities of oil contaminated marine sediment microcosms.

Trending Articles Receiving maximum views in the last few days. Heliobacterium modesticaldum nifH gene. This substrate had the advantage of being completely miscible with the aqueous medium. The novelty of this study is not only related to its methodology, but also to its major findings. Soil bacteria mentioned above are also found in freshwater as they are carried by rainwater runoff, water drainage or via discharge of effluents.

Therefore, these bacteria are useful for the cost-effective, environmentally friendly bioremediation of hydrocarbon contaminants. Microbial biosensors identify and quantify target compounds of interest through interactions with the microbes.

National Center for Biotechnology InformationU. The two general strategies for enhancing oil recovery are altering the surface properties of the interface and using bioclogging to change the flow behavior. Alternatively, oil-mobilizing products can be produced by fermentation and injected into the reservoir. Growth curves were plotted. When further supplied with sufficient limiting nutrients such as nitrogen and phosphor, it grows and produces surfactant glucolipids to help reduce surface water tension and enhance hydrocarbon uptake.