Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.
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Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes. Ahkam SubrotoKian H.
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The We thank Professor T. Methods which eventually showed post-thaw recovery are shown in patterned color. Several transgenic root clones tropane and pyridine alkaloid biosynthetic pathways share a showed 5-fold higher concentrations of scopolamine than the common polyamine metabolism in their early steps. Slow freezing method described by Schmale et al. Freezing program applied was: The hy- few genera of the plant family Solanaceae, including Hyoscyamus, droxylase gene from Hyoscyamus niger has been introduced into Duboisia, Atropa, and Scopolia, are able to produce biologically Atropa belladonna, a typical hyoscyamine-rich tropane alkaloid- active nicotine and tropane alkaloids simultaneously 1—3.
However, scopolamine levels in H lines metabolism existing in most Solanaceae species 1, 7all of the were highly variable, ranging from Method establishment for cryostorage nuticus plant cells is of very empirical nature and often the results do not give clear directions for optimisation work. PCR was performed using h6h gene-specific primers, and it was shown that both transgene and root phenotype responsible rolB region were present in H.
Even though successful cryopreservation methods for a number of plant species have been established, a considerable experimentation is always needed to optimize all the steps in cryopreservation procedure Reed, Cryopreservation of horseradish hairy root cultures by encapsulation-dehydration.
Cryopreservation Several cryopreservation methods were tested for long-term storage of H. D Northern blot analyses for the expression of pmt and h6h in transgenic hairy root lines.
Plant Cell Tissue Organ Cult. B Time courses of growth of five randomly selected hairy root lines. Tips were transferred on fresh solid medium without filter paper after 24 h and incubated in darkness. Genetic stability hyoscumous hairy roots is well recognized.
Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.
Cryoprotection on the other hand covers the use of permeable agents which function through a colligative effect and usually result in the increase in membrane fluidity. Hairy roots, a tumor tissue caused by infection of Agrobacterium rhizogenes is a relevant alternative for plant secondary metabolite production for being fast growing, able to grow without phytohormones, and displaying higher stability than undifferentiated cells.
Here, we report the introduc- tion of gene constructs containing cDNA clones of pmt and h6h, driven by the constitutive cauliflower mosaic virus 35S promoter, into H. The chromatographic conditions for the lines was not reduced as compared with those with low scopol- separation of dansylated N-methylputrescine were as described amine production. Several cryopreservation methods were tested for long-term storage of H.
Chemistry and Pharma- Accumulation of tropane alkaloids and growth of H. The monovalent h6h expression plasmid pLAL21, constructed by Jouhikainen et al.
Plant alka- loids constitute the largest groups of natural mutticus, providing ratio of scopolamine to hyoscyamine in scopolamine-producing cultured roots 9. In this study, hairy roots producing high tropane alkaloid levels were subjected to year follow-up in relation to genetic and metabolic stability. We conclude that transgenic plants harboring both butylbromide form, than for hyoscyamine and atropine com- pmt and h6h possessed an increased flux in the tropane alkaloid bined 9.
The two latter ones do not require special freezing equipment but cells are immersed directly into liquid nitrogen after treatment with hyosfymous solutions or encapsulated with e. We will be provided with an authorization token please note: A positive clone, after confirmation by PCR and cu,ture digestion analysis for the presence of the h6h gene, was used for plant transformation.
This enables the much lowered risk of contamination and improves economic factors related to continuous cultivation. Tropane alkaloid production by hairy roots of Atropa culthre obtained after transformation with Agrobacterium rhizogenes and Agrobacterium tumefaciens containing rol A, B, C genes only. Characterization and stability of tropane alkaloid production during long periods of subculturing.